您的位置:网站首页 > 山茱萸知识 > 正文

山茱萸活性成分能够保护海马神经元

研究发现炮制过的山茱萸抗衰老作用明显,尤其是抗脑部衰老,其活性成分5-羟甲基糠醛对H2O2损伤的大鼠海马神经元具有一定保护作用,可以提高损伤细胞中超氧化物歧化酶的活力,减轻H2O2引起的神经细胞的损伤。

南京中医药大学Mingyan Wang教授带领的团队首次通过细胞生物学和分子生物学手段,探讨山茱萸活性成分5-羟甲基糠醛对氧化损伤的大鼠海马神经元保护作用的分子机制。

发现5-羟甲基糠醛可通过调节凋亡相关基因p53, bcl-2, bax 和caspases的表达拮抗H2O2诱导的大鼠海马神经元凋亡。实验结果为山茱萸活性成分5-羟甲基糠醛防治神经系统退行性疾病提供了理论依据,为临床用山茱萸防治与氧化损伤有关的脑部疾病奠定了基础。

此项研究成果发表在《中国神经再生研究(英文版)》杂志2013年10月第28期。

Previous studies have shown that 5-hydroxymethylfurfural, a compound extracted from wine- pro-cessed Fructus corni, has a protective effect on hippocampal neurons. The present study was de-signed to explore the related mechanisms. Our study revealed that high and medium doses (10, 1μmol/L) of 5-hydroxymethylfurfural could improve the morphology of H2O2-treated rat hippocampal neurons as revealed by inverted phase-contrast microscopy and transmission electron microscopy. MTT results showed that incubation with high and medium doses of 5-hydroxymethylfurfural caused a significant increase in the viability of neuronal cells injured by H2O2. Flow cytometry assays con-firmed that H2O2 could induce cellapoptosis, while high and medium doses of 5-hydroxymethylfurfural had a visible protective effect on apoptotic rat hippocampal neurons. Re-al-time PCR and western blot analysis showed that high and medium doses of 5-hydroxymethylfurfural prevented H2O2-induced up-regulation of p53, Bax and caspase-3 and antagonized the down-regulation of Bcl-2 induced by H2O2 treatment. These results suggested that 5-hydroxymethylfurfural could inhibit apoptosis of cultured rat hippocampal neurons injured by H2O2 via increase in Bcl-2 levels and decrease in p53, Bax and caspase-3 protein expression lev-els.

本文网址: